Laser microdissection of actinomycin D segregated nucleoli.
نویسندگان
چکیده
منابع مشابه
REPOPULATION OF POSTMITOTIC NUCLEOLI BY PREFORMED RNA II . Ultrastructure
The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis . When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as ...
متن کاملRepopulation of Postmitotic Nucleoli by Preformed Rna
The reconstruction of the nucleolus after mitosis was analyzed by electron microscopy in cultured mammalian (L929) cells in which nucleolar RNA synthesis was inhibited for a 3 h period either after or before mitosis. When synchronized mitotic cells were plated into a concentration of actinomycin D sufficient to block nucleolar RNA synthesis preferentially, nucleoli were formed at telophase as u...
متن کاملIntranucleolar Localization of Human Proliferating Cell Nucleolar Antigen pl20*
The human proliferation-associated nucleolar antigen p120 was local ized to substructures within HeLa cell nucleoli by immunofluorescence and immunoelectron microscopy of cells whose nucleoli were segregated by drug treatment or extracted with nucleases. By indirect immunofluo rescence, protein pi 20 was localized diffusely throughout all interphase nucleoli. However, high resolution immunoelec...
متن کاملIntranucleolar localization of human proliferating cell nucleolar antigen p120.
The human proliferation-associated nucleolar antigen p120 was localized to substructures within HeLa cell nucleoli by immunofluorescence and immunoelectron microscopy of cells whose nucleoli were segregated by drug treatment or extracted with nucleases. By indirect immunofluorescence, protein p120 was localized diffusely throughout all interphase nucleoli. However, high resolution immunoelectro...
متن کاملFormation of Microspherules in Nucleoli of Tumor Cells Treated with High Doses of Actinomycin D1 TADAO UNUMA AND HARRIS BUSCH
Microspherules, composed of condensed and segregated fibrillar components, were observed in the nucleoli of the Ehrlich ascites cells and in those of the Novikoff hepatoma cells after treatment with high doses of actinomycin D, but not in Walker tumor cells. The number of these microspherules was calculated to range from 100 to 300 per nucleolus. The density of the microspherules de creased mar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Experimental cell research
دوره 87 2 شماره
صفحات -
تاریخ انتشار 1974